TIMP Details

Crop management
Fine mapping of Quantitative Trait Loci (QTLs) associated with Striga resistant genes in sorghum
  • Kenya
  • Sudan

Description of the technology or innovation

The root hemiparasitic weed Striga hermonthica is a serious constraint to sorghum production in the ECA. Fine mapping of QTLs associated with Striga resistance in sorghum innovation was undertaken to improve the precision of Marker Assisted Selection (MAS) to facilitate breeding of sorghum for striga weed resistance. In this innovation, a total of 169 SSR markers associated with Striga resistance QTLs were identified. Of these, approximately 21 SSRs and 175 DArT markers were found to be closely associated with Striga resistance QTLs and were mapped. Fine mapping is the fastest and currently the most efficient technique for generating evidence for the several combinations of markers or association of markers that support the existence of striga susceptibility genes in the region. Development and evaluation of the striga resistant lines was done in laboratories at ICRISAT and BeCA in Kenya. The innovation has been documented and is ready for up scaling among sorghum breeding laboratories of the ECA. The technology was validated using the base recombinant inbred line (RIL) population and in both cases, the markers linked to the same genomic region confirmed the marker association.

Assessment/reflection on utilization, dissemination & scaling out or up approaches used

The ultimate beneficiaries of this technology are researchers, students and biotechnology companies. Main  method  was  through  publication  of  research  findings  in  journals,  which  can  reach  wider circulation globally among sorghum breeders and researchers.

Current situation and future scaling up

Current extent of reach of intended users of the fine mapping innovation in sorghum breeding

The innovation has not reached other sorghum breeders in the region.

Essential partners/stakeholders) to be involved in the further scaling out/up    
The key partners needed in the scaling up process are:

  • Sorghum breeders  
  • Researchers
  • Students
  • Biotechnology companies

Challenges encountered in respect to further dissemination, adoption and scaling up/out of fine mapping tool

  • This is basic research which calls for awareness among policy makers to facilitate sustained funding
  • Availability of good molecular laboratory facilities
  • Availability of large grounds for field testing

Recommendation for addressing challenges

  • Create awareness among policy makers and end users on fine mapping innovation
  • Forge collaboration linkages with laboratories in advanced countries

Lessons learnt  

  • Need for early awareness by policy makers of the use of this modern tool in the development of striga resistant sorghum varieties.
  • There is need for forge collaboration linkages with advanced laboratories for testing the fine mapping innovations.
  • Social, environmental, policy and market conditions necessary to catalyse fine mapping work
  • Programmes that increase policy makers awareness
  • Conducive institutional mechanisms for variety release

Economic Considerations

Basic  costs  (local  currency  and  equivalent  US  $)  associated  with  utilization  of  the  fine  mapping
innovation  
Not done
Estimated returns (local currency and equivalent US$) such as cost benefit or gross margin figures
where applicable
Not done

Gender considerations

Gender issues /concerns (if any) were considered in the fine mapping exercise
Was not part of the study
Gender issues/concerns  (if any) in the fine mapping exercise
Was not part of the study
Gender-related opportunities (if any), which enhanced or can enhance the genetic linkage mapping  
Was not part of the study

Case study or profiles of success stories

Success stories from beneficiaries attesting to the use of the genetic linkage mapping
None as yet

Application guidelines for the users

Methodology for development genetic linkage map  
Are well documented and are being published

Contact details

Mohamed H Abdalla
Associate Professor, Agricultural Research Cooperation (ARC)
P.O Box 126
Khartoum, Sudan
Mob: +249-921934900
Email: abdalla_moh2002@yahoo.com; abdallamoh2002@gmail.com


Dr. Rasha Ali
ASARECA TIMPS-Revised Nov 2014  Page 51
Associate, Agricultural Research Co-operation (ARC)
P.O Box126
Khartoum, Sudan
Email: yarashaya@gmail.com


Dr Tom C. Hash
Sorghum Breeder
ICRISAT
Pantecheru,
India
Email: c.harsh@cgiar.org


Dr Santie de Villiers
Senior lecturer
Pwani University
Department of Chemistry and Biochemistry
PO Box 195-80108
Kilifi, Kenya
Tel: +254 729369758
E-mail: s.devilliers@pu.ac.ke
santiedevilliers@gmail.com


Dr. Dionysious (Dan) Kiambi
Executive Director
African Biodiversity Conservation and Innovations Centre (ABCIC)
P.O. Box 100882-00101, Nairobi, Kenya
Tel/Fax. 254-20-2330014
Mobile.254-731823394/704417920
Email:d.kiambi@abcic.org

Additional information

ANNEX 1

Saturation of Sorghum Linkage Map with SSR Markers

ANNEX 2
Glossary of terms
ARC:     Agricultural Research Cooperation of Sudan
ECA :    Eastern and Central Africa
MAS:     Marker Assisted Selection  
QTL:     Quantitative Trait Loci